克日，壹定发生物自主研发的创新手艺再ci获得国际认可，包罗全内反射荧光成像系统在内的三项发现专利正式通过欧洲专利局（European Patent Office）的授权批准，成为有用欧洲专利。
至此，历经多年自主研发积累，壹定发生物现在拥有的海内外专利（含授权有用和申请中）及盘算机软件著作权挂号等已近200件。
01
METHOD FOR CONTROLLING BASE SEQUENCE DETERMINATION?REACTION,?BASE SEQUENCE DETERMINATION SYSTEM AND CONTROL DEVICE
（对序列测定反映举行控制的要领、序列测定系统和控制装置）
手艺简介：
序列测定系统包罗流体装置和光学装置，反映装置包罗第一单元和第二单元，界说序列测定反映包罗的一种重复执行单元为第二生化反映-第一生化反映-照相，要领包罗在完成初shi步骤之后，使适当使用流体装置使第一单元和第二单元中的一个上的样品举行第二生化反映和第一生化反映的同时，使用光学装置对另一个单元上的样品举行照相。初shi步骤包罗：a）使用流体装置使第一单元和第二单元中的一个举行样品的第一生化反映，b）使用光学装置对举行第一生化反映后的单元的样品的举行照相，c）使用流体装置使第一单元和第二单元的另一个举行样品的第一生化反映。上述要领可提高序列测定效率。
“The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.”
02
SINGLE-MOLECULE IMAGE CORRECTION METHOD, DEVICE AND?SYSTEM, AND COMPUTER-READABLE STORAGE MEDIUM
（单分子图像纠偏要领及装置）
?
手艺简介：
本专利果真了一种单分子图像纠偏要领及装置，其中要领包罗：凭证第一图像的每一像素点对应的亮度值矩阵，获取与所述第一图像对应的第一目的简化矩阵，并凭证第二图像的每一像素点对应的亮度值矩阵，获取与所述第二图像对应的第二目的简化矩阵；对所述第一目的简化矩阵和所述第二目的简化矩阵划分举行二维离散傅里叶变换，获得与所述第一目的简化矩阵对应的第一傅里叶矩阵，并获得与所述第二目的简化矩阵对应的第二傅里叶矩阵；凭证所述第一傅里叶矩阵和所述第二傅里叶矩阵获取所述第二图像相对于所述第一图像的偏移量；凭证所述偏移量对所述第二图像举行纠偏。可见，实验本发现能够对差异时刻拍摄的单分子图片举行纠偏。
“An embodiment of the present disclosure discloses a method and a device of correcting a single-molecule image, the method includes: acquiring the first target simplified matrix corresponding to the first image according to the intensity matrix corresponding to each of pixels in the first image, and acquiring the second target simplified matrix corresponding to the second image according to the intensity matrix corresponding to each of the pixels in the second image; the two-dimensional DFT is performed to the first target simplified matrix and the second target simplified matrix respectively to acquire the first Fourier matrix corresponding to the first target simplified matrix and the second Fourier matrix corresponding to the second target simplified matrix; the method of correcting a single-molecule image acquire the offset of the second image with respect to the first image according to the first Fourier matrix and the second Fourier matrix; and correcting the second image according to the offset. Such method can correct the images of single molecules taken at different moments.”
03
TOTAL INTERNAL REFLECTION FLUORESCENCE IMAGING SYSTEMAND?SEQUENCING DEVICE
（全内反射荧光成像系统及测序装置）
手艺简介：
本专利果真了一种全内反射荧光成像系统及测序装置，全内反射荧光成像系统包罗第一成像系统和第二成像系统，所述第一成像系统包罗N条自力的激光光路，所述第二成像系统通过第二吸收装置获取到第二像的位置的差异判断样品高度的转变，凭证样品高度的转变举行自动赔偿，襶uanＶぱ穝hi终处于聚焦的位置。本发现可通过第二成像系统划分对N条自力的激光光路举行光路调治，以实现N条自力的激光光路具有相同的穿透深度，进而无需在切换差异光源时，再举行光路调治，从而节约了实验当中费时的光路调治。
“The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment.”
自主研发、科技创新是壹定发建设之初的内在基因，也是壹定发一连生长至今的战略基本。为了有用支持公司科技创新战略，包罗有用地对无形资产举行转化和治理运营，公司一直承袭尊重科学家和工程师的团队文化，并据自shenqing况设立专门部门和人yuan举行知识产权治理，公司知识产权的治理运行切合国家推荐衴uan曜糋B/T 29490-2013《企业知识产权治理规范》。未来，壹定发将进一步增强深化知识产权结构，一连促进公司焦点竞争力的稳步提升。